A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
hFetuin B(1-382)-Fc-6xHis and hOvastacin(1-431)-twinStrep purification: Nickel & Strep-Tactin®T 4Flow® high capacity FPLC column For relevant background please see relevant page: Assessing Ovastacin and Fetuin B as a Non-Hormonal Contraceptive Targets (https://openlabnotebooks.org/assessing-ovastacin-an-fetuin-b-as-a-non-hormonal-contraceptive-targets/) Donor Plasmid: pFastBac-Dual vector was used for the dual expression of hOvastacin-twinStrep (driven by polyhedrin promoter) and hFetuin B-Fc-6xHis (driven by p10 promoter). Recombinant Read More …
The Recap It’s been one year since the last update on the α/β hydrolase domain 2 (ABHD2) as a target for non-hormonal contraception (see my previous post here). The major challenges were the production of ABHD2 and development of a reliable activity assay to screen and quantify inhibitory compounds. At that point 14 different constructs Read More …
Pyrazole (E)-vinyl sulfone 3 was identified as a potential covalent CHIKV nsp2 protease inhibitor from a high throughput screen in the READDI Antiviral Drug Discovery Center at UNC.1 Initial re-synthesis of the hit compound yielded a mixture of acyclic (3) and cyclic sulfones (4) (Scheme 1), which were identified by 1D and 2D NMR, but Read More …
High throughput screening (HTS) is a powerful technique for identifying potential drug candidates from large libraries of compounds. However, many compounds that show activity in HTS are false positives or may not be suitable for further development. Therefore, it is important to apply various filters to eliminate compounds that have undesirable properties, such as poor Read More …
The Structural Genomics Consortium (SGC) has been funded by the Bill & Melinda Gates Foundation to research Women’s and Children’s Health, focusing on the advancement of drug discovery in reproductive biology and disease, child development, and childhood diseases. The SGC plans to generate protein reagents and chemical probes for several potential drug targets for new, Read More …
Figla test expression #4
Test of Monoclonal and Polyclonal Ab
Figla test expression #3
