SGC Univ. of Frankfurt

Co-expression and purification of recombinant hOvastacin and hFetuin B in insect cell sf9

Posted onAuthorYufeng PanLeave a comment

hFetuin B(1-382)-Fc-6xHis and hOvastacin(1-431)-twinStrep purification: Nickel & Strep-Tactin®T 4Flow® high capacity FPLC column For relevant background please see relevant page: Assessing Ovastacin and Fetuin B as a Non-Hormonal Contraceptive Targets  (https://openlabnotebooks.org/assessing-ovastacin-an-fetuin-b-as-a-non-hormonal-contraceptive-targets/) Donor Plasmid: pFastBac-Dual vector was used for the dual expression of hOvastacin-twinStrep (driven by polyhedrin promoter) and hFetuin B-Fc-6xHis (driven by p10 promoter). Recombinant Read More …

Assessing Ovastacin and Fetuin B as a Non-Hormonal Contraceptive Targets

Posted onAuthorGrant MillsLeave a comment

Background: Ovastacin is an oocyte-specific protease involved in sperm adhesion and fertilization. Research into the ovastacin’s protease function suggest it acts on the post-fertilization cleavage of ZP2 via cortical granule exocytosis1. Cleaved ZP2 then causes hardening of the zona pellucida and is then unable to support further sperm binding2. Zona pellucida regulation is critical for Read More …