A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Hi all, it’s been a while since my last post; I’ve been busy in the lab purifying new protein constructs, developing a new assay and expanding the chemical series of my weak binding hits for USP5 ZnF-UBD, so there will be a couple of posts coming your way in the following weeks. This week, my Read More …
Synopsis The four, structurally similar Mur ligases are essential for the survival of gram-negative bacteria and are therefore potential targets for new antibiotics. Crystallographic fragment screening may provide new avenues for inhibitor development. Background The rapid emergence of bacterial resistance to almost all clinically available antibiotics is one of the biggest healthcare challenges of Read More …
I joined the group of Dr. Cheryl Arrowsmith 2 months ago. Currently, I am working on the Huntington’s disease project along with Dr. Rachel Harding and Jacob McAuley. During these past two months, I have been learning how to purify huntingtin (HTT) protein and overall getting up to date with the project. A brief introduction Read More …
As an Irishman, I am well versed in roundabouts, we love them. Entering the roundabout, going around it and then hoping to get off it when its your exit. If you don’t exit, you just keep going round and around and around, well you get the drift. It reminds me of that great scene in Read More …
In the immortal words of Ron Burgundy “Its so hot… milk was a bad choice”. Man, it is a scorcher today, with a humidex of really humid, now I know how cells feel like living in an incubator. But when its hot, what better way to cool down then with an ice cold beverage (I Read More …
The Intelligent Assistive Technology and Systems Lab (IATSL) is a multi-disciplinary group of researchers with backgrounds in engineering, computer science, occupational therapy, speech-language pathology, and gerontology. Our goal is to develop zero-effort technologies that are adaptive, flexible, and intelligent, to enable users to participate fully in their daily lives. Our research primary focuses on older Read More …
In my last post, I told you about my initial results soaking follow-up compounds into preformed HAO1 crystals. In order to generate more crystals (and so more structures), I purified some new protein (I’ll call it ‘batch #2’ for simplicity). However, this second batch, when crystallised, did not take up any of the soaked in Read More …
Good morning and how is everyone doing, enjoying that first cup of tea or coffee of the morning I hope. Myself, I am a tea person, always have been, my Irish nature, we drink it by the liter. Can’t beat a good of tea in the morning to revive oneself. And if it’s the afternoon Read More …
More forgotten experiments revived by my year-end report. Huntingtin chromatin retention is reduced in PARP KO cells, but in frustration over some confusing results, I overlooked this important data. Lesson: pause and look at the data before rushing on! All the details and links to Zenodo deposits can be found on the Truant lab website.
Background: Aside from determining the optimal numbers of DIPG cells to be seeded for viability assay, it is also important to validate that measurements using CellTiter Glo are in agreement with other methods of determining cell viability. Experimental design: I chose to compare the EC50 values of M4K2009 on HSJD-DIPG-007 when measured using different methods. Read More …
