A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Background The SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase is a complex of proteins that helps facilitate the ubiquitination of substrates for targeted proteasomal degradation. FBXW11 is F-box containing protein that has 11 WD-repeats and is a substrate recognition component of the SCF complex. FBXW11 recognizes and binds its substrates, including beta-catenin, at phosphorylated sites or Read More …
Background The SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase is a complex of proteins that helps facilitate the ubiquitination of substrates for targeted proteasomal degradation. FBXW7 (F-box/WD repeat-containing protein 7) is an F-box protein component of the SCF complex and is involved in substrate recognition. The F-box domain tethers FBXW7 to the SCF complex by interacting Read More …
Please see FBXW7-349-707_Nhis Expression and Purification protocol in the link below: https://zenodo.org/record/4118423
Below is the link for expression and purification TEAD4:212-434 protocol on Zonodo.https://doi.org/10.5281/zenodo.4116056
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant NSD3 PWWP1 for further research. You can read more details on Zonodo in link below: https://doi.org/10.5281/zenodo.4115939
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant NSD2 PWWP1 for further research. You can read the purification protocol on zenodo.
Production and purification of recombinant SARS COV-2 RBDSD1-Fc protein from a transiently transfected EXPI293 mammalian cells. Expression plasmid for CoV-9-RBDSD1, residues of RBD R319−S591 with a C-terminal HRV3C protease cleavage site, a monomeric Fc tag and an 8XHisTag was generously gifted by Dr. McLellan’s lab (1). Secreted proteins were purified from a suspension culture cell Read More …
Over the last few months, we have all become familiar with the COVID-19 global pandemic. The coronavirus is “decorated” with the Spike (S) protein, one of the 25 proteins of SARS-CoV-2 that are encoded in the viral genome. The infection starts with the attachment of the virus to the cell membrane to enter host cells. Read More …
RBBP4 and RBBP7 (RBBP4/7) are highly homologous nuclear WD40 motif-containing proteins widely implicated in various cancers and are valuable drug targets. Below is the link to the expression and purification of the protocol for RBBP7. RBBP7 protein is used to develop drug screening assays towards the discovery of novel cancer therapeutics. https://zenodo.org/record/4086006#.X4ckLWhKi70
RBBP4 and RBBP7 (RBBP4/7) are highly homologous nuclear WD40 motif-containing proteins widely implicated in various cancers and are valuable drug targets. Below is the link to the expression and purification of the protocol for RBBP4. RBBP4 protein is used to develop drug screening assays towards the discovery of novel cancer therapeutics. https://zenodo.org/record/4085998#.X4cfCmhKi70
