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Background The SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase is a complex of proteins that helps facilitate the ubiquitination of substrates for targeted proteasomal degradation. FBXW11 is F-box containing protein that has 11 WD-repeats and is a substrate recognition component of the SCF complex. FBXW11 recognizes and binds its substrates, including beta-catenin, at phosphorylated sites or Read More …
Background The SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase is a complex of proteins that helps facilitate the ubiquitination of substrates for targeted proteasomal degradation. FBXW7 (F-box/WD repeat-containing protein 7) is an F-box protein component of the SCF complex and is involved in substrate recognition. The F-box domain tethers FBXW7 to the SCF complex by interacting Read More …
Background HSP70 heat-shock proteins family, including constitutively expressed HSPA8 variant, facilitate correct folding of client proteins and degradation of unfolded clients. To achieve folding or degradation of client proteins, HSP70 cooperates with co-chaperones, which mostly bind to the highly conserved sequence EEVD in the C-terminal domain of HSP70 via their tetratricopeptide repeat (TPR) domain (PMID: Read More …
Background Apart from my HTT protein project, I also work on UBE3A, a HECT domain E3 ligase. E3 ligases work as a part of the ubiquitination system interacting with E2 ubiquitin-conjugating enzymes to transfer ubiquitin onto target substrate proteins. Ubiquitination of substrate proteins regulate a diversity of cell processes and is also important for ubiquitin-mediated Read More …
Background RFWD3 (E3 ligase RING finger and WD repeat domain-containing protein 3) and RPA2 (Replication protein A2) were shown to functionally interact and participate in replication checkpoint control. At stalled replication forks, the RPA complex binds single-stranded DNA to prevent nucleolytic digestions and also to initiate replication checkpoint signaling by recruitment checkpoint proteins. RFWD3 is Read More …
Background GID4 is the substrate-recognition subunit of the CTLH E3 ubiquitin-protein ligase complex which has been shown to possess E3-ligase enzymatic activity (PIMID:31885576). GID4 was shown to bind proteins and peptides with a Pro/N-degron consisting of an unmodified N-terminal Pro and has the highest affinity for the peptide Pro-Gly-Leu-Trp (PIMID:29632410). In yeast Gid4 specifically targets Read More …
Background PRMT4 (CARM1) is type I protein arginine methyltransferase which mono- or asymmetrically dimethylates proteins (Fig.1) within proline-methionine rich motives, that are involved in transcription, splicing and RNA processing such as H3R17, EP300, PABP1, CA150, BAF155, MED12 (PIMID:19150423). Fig.1. Mammalian PRMTs. There are 9 members and 3 types of the PRMT family. Type I, II Read More …
