Month: January 2019

Welcome to the SGC-Karolinska side of ALS-RAP: the scientists, the screening, and the antibody selections

Posted onAuthorCarolyn MarksLeave a comment

Hello fellow scientists and open lab notebook readers! I’m Carolyn Marks and I’m working in the ALS-RAP partnership with Susanne Gräslund, who leads the Recombinant Antibodies Group at SGC-Karolinska in Stockholm, Sweden. SGC-Karolinska has had a long-standing collaboration with Helena Persson Lotsholm, Director of the Human Antibody Therapeutics Facility within the Drug Discovery and Development Read More …

Testing Hit Analogues against USP5 Zf-UBD with SPR Assay #1

Posted onAuthorMandeep MannLeave a comment

In a previous post, I described using hit expansion tools such as commercial substructure searches, docking, and free energy perturbation (FEP) simulations to explore the structure activity relationship (SAR) of the chemical series focused on the Zf-UBD of USP5. The first batch of commercially ordered compounds arrived just before the Christmas holidays. I decided to Read More …

Optimising seeding of normal and FOP cells for a later assay – prettier than expected

Posted onAuthorElizabeth Brown1 Comment

Further down the line I want to be able to test the activation of the BMP signalling pathway downstream of the ALK2 receptor. For this I plan to use the alkaline phosphatase gene as a readout – it’s a well known gene activated downstream of BMP signalling so a lot of easy to use assays Read More …

Further Investigation of the Reduction of HTT Protein through Chemical Inhibition

Posted onAuthorJolene HoLeave a comment

The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.2541053. A lot has happened in the past few months and it is with mixed feelings that I am finishing up my postdoc here at SGC! I feel very privileged to have been part of the great science going on at SGC Read More …

More crystals through XChem

Posted onAuthorJose Brandao-NetoLeave a comment

Following an early commissioning stage, XChem crystallised its core xray collection protocol as 180 degrees rotation in 60 seconds, which provides PanDDA with good datasets. But what has never been fully characterised is the lowest dose per crystal required for PanDDA to work. XChem now has an established hit rate profile where, based on our Read More …

A new ALK2 structure, and proof that mistakes in the lab aren’t always a bad thing

Posted onAuthorRoslin AdamsonLeave a comment

Hello everyone and happy New Year! I really, really wanted to end the year on a high note in December before I went off on holiday, but as it turned out, I didn’t have enough time to set up 18 plates and write a blog post on the last day. So I’ll just have to Read More …