A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
In my previous post, I shared the expression and purification results of several constructs based on bacterial expression systems. Such experiments often fail as there are inherent complications with protein expression associated with bacterial expression systems. This was likely the case for most of our last batch samples. To tackle this problem, we decided to Read More …
Recombinant protein expression is a prominent molecular technique for structural biology studies. For expressing recombinant proteins, a host organism is utilized as protein production factories. To obtain the desired recombinant proteins, we first design a recombinant DNA, which involves the information of protein to be produced. Then, we clone the desired DNA fragments into a Read More …
The molecular blueprint of the living organisms, deoxyribonucleic acid (DNA), is often wrapped with a family of proteins, called histones, in mammalian cells. The functions of histones are vast, including the protection of DNA, and most importantly, the regulation of gene expression. However, the details of gene regulation function of histones are not clearly understood, Read More …
