A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
After a long time away from the lab, it’s nice to have some new and interesting data! Details here: https://raytruantlab.ca/2021/02/11/huntingtin-coats-mitotic-chromosomes-when-par-break-down-is-inhibited/
In my previous purification of the HTT N-HEAT_81-1643 construct, I showed that an additional heparin step did not remove any traces of nucleic acid material, changed purity or oligomeric state of the sample. Additionally, my DLS results showed that this construct forms large particles in solution. Thus, the DLS results in combination with the gel filtration Read More …
In the second purification round of N-HEAT_81-1643, I added an additional purification step with heparin resin. My aim with doing this additional step was to improve the purity of the HTT N-HEAT_81-1643 sample from nucleic acid material/other proteins. Similar experiments proved successful for full HTT+HAP40 sample (by Dr. Rachel Harding). Our results showed that the Read More …
Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
A few recent experiments that have been deposited to Zenodo can be found here: https://raytruantlab.ca/2019/12/02/1927/
Five months ago, I made my first post outlining the goals of my project here at the SGC. I am writing again today to update you on the progress I have made toward those goals. As a brief overview, I was working on finding Huntingtin (HTT) interacting proteins, but why would this be important? If Read More …
It’s being a while since I wrote my first post. I have been busy in the lab trying to isolate monomeric and stable samples of the N-HEAT domain just as we demonstrated with the C-HEAT domain (and by Dr. Rachel Harding). In this post, I summarize the results of the first round of purification of Read More …
One of the major findings of the HDSA-funded project to find huntingtin interactors relevant DNA repair was that many proteins that interact with huntingtin are modified by poly ADP ribose (PAR). Previous posts have described our hypothesis that huntingtin binds poly ADP ribose, our preliminary evidence that it does so, frustrating attempts to understand why it Read More …
Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
Summer travels showcasing the huntingtin protein toolkit
