A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Synopsis The four, structurally similar Mur ligases are essential for the survival of gram-negative bacteria and are therefore potential targets for new antibiotics. Crystallographic fragment screening may provide new avenues for inhibitor development. Background The rapid emergence of bacterial resistance to almost all clinically available antibiotics is one of the biggest healthcare challenges of Read More …
Following an early commissioning stage, XChem crystallised its core xray collection protocol as 180 degrees rotation in 60 seconds, which provides PanDDA with good datasets. But what has never been fully characterised is the lowest dose per crystal required for PanDDA to work. XChem now has an established hit rate profile where, based on our Read More …
The year is coming to an end and I want to start my blog by giving you an overview of a project that kept me busy for the better part of it. Our existing Protein Data Bank (PDB) batch deposition mechanism underwent a major overhaul recently and we are finally in a position where we Read More …
Takeaway: Why: we need lots of well diffracting crystals. How: crystallisation growth optimisation. What: tweaking conditions, phase space understanding, seeding, MMS. At this day and age (2018) many people are thinking that the future of structural molecular biology being about serial XFEL or synchrotron crystallography and electron diffraction, which are great tools to harness the Read More …
