Month: March 2023

Recombinant Expression and Purification of ABHD2 from E. coli

Posted onAuthorMadison EdwardsLeave a comment

His6-FLAG-ABHD233-425 (PBC042 A11) Purification: Nickel & Size Exclusion Chromatography  This post is also available at: Recombinant Expression and Purification of ABHD2 from E. coli | Zenodo DOI: 10.5281/zenodo.7696914 For relevant background please see relevant page: A promising target for non-hormonal contraception – The a/b Hydrolase Domain 2 | Zenodo Expression of ABHD2 For each 1.8 Read More …

Exploring the Potential of Human Helicase Ligands as Inhibitors for SARS-CoV-2 NSP13 Pt. 2

Posted onAuthorLakshi SelvaratnamLeave a comment

My goal is to look for strategies and chemical starting points to inhibit viral helicases, with a primary focus on the SARS-CoV-2 helicase NSP13. In a previous post, I showed that a binding pocket in the human helicase SNRNP200 exploited by an allosteric inhibitor (PDB code 5URK) was absent in SARS-CoV-2. Here, I analyze another Read More …