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Background Apart from my HTT protein project, I also work on UBE3A, a HECT domain E3 ligase. E3 ligases work as a part of the ubiquitination system interacting with E2 ubiquitin-conjugating enzymes to transfer ubiquitin onto target substrate proteins. Ubiquitination of substrate proteins regulate a diversity of cell processes and is also important for ubiquitin-mediated Read More …
In my last blog post, I described how I am trying to overexpress HTT in neuronal cells. While the BacMAM system was easy to implement in cells such as HEK293, it proved far more challenging to express in SH-SH5Y neuronal cells. Since then, I have attempted to use the BacMAM system in two other neuronal Read More …
My main project revolves around the E3 ligase UBE3A (I will be writing a project overview for this later), but I am also continuing some work in the same vein as Jolene Ho’s work looking at role of HTT in neurological disorders. HTT encodes a protein called huntingtin whose function is not known but appears Read More …
