Recombinant Expression and Purification of ABHD2 from E. coli

His6-FLAG-ABHD233-425 (PBC042 A11) Purification: Nickel & Size Exclusion Chromatography  This post is also available at: Recombinant Expression and Purification of ABHD2 from E. coli | Zenodo DOI: 10.5281/zenodo.7696914 For relevant background please see relevant page: A promising target for non-hormonal contraception – The a/b Hydrolase Domain 2 | Zenodo Expression of ABHD2 For each 1.8 Read More …

Validated antibodies for ALS research: the ALS-RAP project

We are part of ALS-RAP, the ALS (Amyotrophic Lateral Sclerosis) Reproducible Antibody Platform. We’re addressing one of the big conundrums to biomedical research: the lack, or the poor quality, of many antibodies used for research ranging from cell biology to histopathology. ALS, also called MND (motor neurone disease), is a group of diseases that cause Read More …

Introducing the team: The Gileadi Group in Oxford.

Hi! I’m Opher Gileadi, I head a group at the Structural Genomics Consortium (SGC) in Oxford. For many years, we’ve been looking at proteins involved in human. We’ve looked at a wide variety of proteins: from DNA repair enzymes and transcription factors to enzymes involved in signalling. In most cases, we focus on early work: Read More …