A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Hi, Recently, I have started working on NanoBret PPI technology for chromatin factors and E3 ligases are here is one of the assays I developed. Background (source: PMID28179136). The repressive Nucleosome Remodeling and histone Deacetylation (NuRD) complex alters chromatin structure by coupling ATP-dependent remodeling activity with histone deacetylation function. It is composed of six different Read More …
Hi Here are the details of the assay I developed to measure interaction between USP5 and ubiquitin in cells. Ubiquitin specific peptidase 5 (USP5) belongs to the largest ubiquitin-specific protease (USP) subfamily and has a zinc finger at its N-terminal portion and two ubiquitin-associated domains in the large catalytic region that mediate polyubiquitin binding (PMID:28923280, Read More …
Hi all! Hope everyone is staying safe and practising social distancing during these difficult times. Working from home, I’ve decided to catch up on my open notebook. In a previous post, I purified various full length USP5 constructs and determined the binding affinity of ubiquitin to these constructs. I wanted to determine the stoichiometry of Read More …
In a previous post, we solved the co-crystal structure of the USP5 zinc finger ubiquitin-binding domain (ZnF-UBD) with a promising compound, UBTR012574, which had a potency of approximately 10 µM (Figure 1). W expanded the chemical series of UBTR012574 by docking analogue compounds from the Enamine REAL database and Scifinder. We decided to order 17 Read More …
When I was a kid, I used to have a spinning globe lamp in my bedroom. What can I say? I was a big nerd child (I also had a passion for stamp collecting and making model airplanes, but thats a tale for another time). I loved plugging it in and when it lit up Read More …
