A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Nature has evolved molecules called glycoproteins that are proteins carrying sugar chains (“glycans”). Glycans can make proteins more soluble, they can shield them from the environment, they can be used as calling cards to enable protein-protein recognition; in Eukaryotes, a glucose-on/glucose-off quality control system uses glycans to monitor glycoproteins folding. In general, glycans contribute to Read More …
A summary of the Berman Topper second year-end progress report, with links to the full report, are now posted on the Truant lab website.
Access to large amounts of core building blocks is essential in drug discovery programs. It enables rapid synthesis of a series which can then give structure activity relationships (SAR) of the drugs and their target. SAR data allows medicinal chemists to determine the group(s) in a structure evoke a change in activity, whether positive or Read More …
Defining Epigenetic Drivers of Medulloblastoma – Introduction Medulloblastoma is a cancer that arises from neural progenitor cells in the brain. This cancer most commonly occurs in young children ages 1-4, and is the most common type of childhood brain cancer. Although many cases of medulloblastoma show good prognosis, there are some sub-types of this cancer Read More …
Sorry I’ve not written for a while. I had some annual leave to use up and then I’ve been helping out with a residential EMBO course that was being co-organised by one of the group leaders in my department. The course was an introduction to a wide variety of biochemical and biophysical techniques. I was Read More …
As the summer continues to progress, so does my project. After the last round of purification, detailed in my previous blog post, I am ready for another round. During this purification, I will be working with SH3G3 and PACN1. PACN1 is responsible for cytoskeletal remodeling through regulation of several other proteins, as well as neuronal Read More …
As explained in my previous post [https://openlabnotebooks.org/2714-2/], my goal is to test whether structure-based approaches can guide the design of PROTACs. As a first step, I am evaluating whether protein-protein docking tools can accurately predict the interface between an E3 ligase and its target, a question recently explore by Drummond and Williams.1 This step is Read More …
In my last post, I reported a new synthetic route to our quinoline series, Scheme 1. The goal was to overcome prior synthetic challenges and to allow for rapid synthesis of many different analogs over a short period of time. Scheme 1: New synthetic route for generating quinoline analogs I have as a result generated large Read More …
To start off my project at the SGC, my supervisor, Dr. Rachel Harding, and I produced and purified three proteins. These proteins were RAB1A, RAB1B, and MECP2. Both RAB1A and RAB1B have functions in the initiation of autophagy, which is a cellular system that helps clean out old debris and malfunctioning parts. MECP2 is a Read More …
You may recall I was in Montreal for the last three months doing an internship with Molecular Forecaster Inc. (MFI), a computational chemistry company. I’m back in Toronto from my internship, ready to jump back into my research, but first I’ll summarize the virtual screening work I did at MFI for USP5. In a previous Read More …
