After a long time away from the lab, it’s nice to have some new and interesting data! Details here: https://raytruantlab.ca/2021/02/11/huntingtin-coats-mitotic-chromosomes-when-par-break-down-is-inhibited/
A few recent experiments that have been deposited to Zenodo can be found here: https://raytruantlab.ca/2019/12/02/1927/
One of the major findings of the HDSA-funded project to find huntingtin interactors relevant DNA repair was that many proteins that interact with huntingtin are modified by poly ADP ribose (PAR). Previous posts have described our hypothesis that huntingtin binds poly ADP ribose, our preliminary evidence that it does so, frustrating attempts to understand why it Read More …
More forgotten experiments revived by my year-end report. Huntingtin chromatin retention is reduced in PARP KO cells, but in frustration over some confusing results, I overlooked this important data. Lesson: pause and look at the data before rushing on! All the details and links to Zenodo deposits can be found on the Truant lab website.
A short report on a successful method of detecting poly ADP ribose by immunofluorescence has now been posted on the Truant lab website.
A summary of the Berman Topper second year-end progress report, with links to the full report, are now posted on the Truant lab website.
Updates on the huntingtin PAR binding story can be found on the Truant lab website.
Hypothesis: Poly ADP ribose signaling is dysregulated in Huntington’s disease
Is huntingtin a PAR-binding protein?
A closer look at the hits generates 2 new hypotheses