A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
The Recap It’s been one year since the last update on the α/β hydrolase domain 2 (ABHD2) as a target for non-hormonal contraception (see my previous post here). The major challenges were the production of ABHD2 and development of a reliable activity assay to screen and quantify inhibitory compounds. At that point 14 different constructs Read More …
His6-FLAG-ABHD233-425 (PBC042 A11) Purification: Nickel & Size Exclusion Chromatography This post is also available at: Recombinant Expression and Purification of ABHD2 from E. coli | Zenodo DOI: 10.5281/zenodo.7696914 For relevant background please see relevant page: A promising target for non-hormonal contraception – The a/b Hydrolase Domain 2 | Zenodo Expression of ABHD2 For each 1.8 Read More …
The Background In the scope of the SGC’s (Structural Genomics Consortium) Women’s and Children’s Health Initiative, a group of proteins that are potential targets for new, safe, and effective non-hormonal contraceptives will be characterized. These will then used as a template to screen for possible chemical probes and drug candidates. A group of postdoctoral researchers Read More …
Hello fellow scientists and open lab notebook readers! I’m Carolyn Marks and I’m working in the ALS-RAP partnership with Susanne Gräslund, who leads the Recombinant Antibodies Group at SGC-Karolinska in Stockholm, Sweden. SGC-Karolinska has had a long-standing collaboration with Helena Persson Lotsholm, Director of the Human Antibody Therapeutics Facility within the Drug Discovery and Development Read More …
