As mentioned in my last post, I got 26 mg of ACVR1 on my last purification (as shown in my Zenodo log here). This is fabulous as it has allowed me to follow up a number of conditions for crystallisation. In particular, one of these hits was in G10 of my composite screen. I’ve therefore set up a lot of plates around this condition, and at the same time have mounted some of the crystals that were in those wells with different compounds, and will be screening them at the synchrotron tonight. The best outcome of course would be a dataset, but at least if they diffract ok I know I have something to work with, as the fine screens are showing some small, needly crystals which could probably be optimised a bit.
Wish me luck, and I’ll hopefully update on Monday!