A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1495129. Apologies for the long delay between this post and my last post, it really has been quite some time! As postdocs, we generally have several projects running simultaneously and we have to split our time between these projects. I am no Read More …
Huntington Society of Canada 2018 Conference in Kelowna
Hypothesis: Poly ADP ribose signaling is dysregulated in Huntington’s disease
Biophysical investigation of HTT and HTT-HAP40 using DSLS and SAXS
The experimental set-up and results covered in this post can be found here: doi.org/10.5281/zenodo.1420537 I was previously using an HTT overexpression model to look at any changes in proteins related to HTT or its function. Unfortunately, none of the related proteins I looked at showed any significant change in protein levels with HTT overexpression. There Read More …
Is huntingtin a PAR-binding protein?
Searching far and wide for proteins that bind huntingtin
Expression and purification of the HTT-HAP40 complex with expanded polyQ (Q54)
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1323902 As I’ve been having problems overexpression HTT properly in my cancer cell lines, I decided to continue looking at other proteins of interest using our best overexpression model so far, which is in HEK293T cells. Although it may not be a cancer Read More …
A closer look at the hits generates 2 new hypotheses
