Previously, we established that OCI-AML-20 requires stroma to maintain CD34 expression (https://zenodo.org/record/1187083#.Wr5-1C7wbIV). In this experiment, we wanted to see whether those cells grown in suspension are able to regain CD34 expression when cells are reintroduced to stroma. We find that OCI-AML-20 regains CD34 expression when put back on to stroma (either MS5 or OP9). Full experimental details can be found at Zenodo.
Figure 1: Summary of flow cytometry analysis of cells at 4 weeks. Cells that were transferred from suspension to stroma regain CD34 expression, and for CD34+CD38- we see expression levels seen with cells grown continuously on stroma.