A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Friday, August 9th, 2019. This date has been etched on my calendar since early June. For many of you, this is just a regular Friday in August, the end of a working week and the start of the weekend, nothing overly special about it. But as a Liverpool FC fan, this date represents the end Read More …
…and analysing their activity! Following on from the work I talked about here I’ve been able to start looking at the influence the different phosphorylation sites have over the ability of ALK2 to activate SMAD1. Just coming through the SGC expression pipeline are several constructs containing mutations in various combinations of the sites known to Read More …
Well hello there, did you miss me. No, I didn’t get stuck on a roundabout for days, preventing me from writing my next blog. I was simply enjoying the Montreal sunshine and those umbrella-containing beverages. Whoever came up with the idea of an umbrella in a drink is a genius. The umbrella shades the drink, keeping Read More …
My main project revolves around the E3 ligase UBE3A (I will be writing a project overview for this later), but I am also continuing some work in the same vein as Jolene Ho’s work looking at role of HTT in neurological disorders. HTT encodes a protein called huntingtin whose function is not known but appears Read More …
I am very excited to write my first post in open notebook. My post on Zenodo summarizes the results of my first ever purification of a huntingtin (HTT) domain. I was able to purify successfully the HTT C-HEAT domain encompassing residues 2088-3144. Figure 1. Anti-FLAG purification of the HTT C-HEAT domain. Eluted protein is seen Read More …
