A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.2541053. A lot has happened in the past few months and it is with mixed feelings that I am finishing up my postdoc here at SGC! I feel very privileged to have been part of the great science going on at SGC Read More …
In a previous post, I described the importance of solving the structure of other USPs that have a Zf-UBD for the rational design of selective compounds. The last post described the design, cloning, E.coli expression, growth and purification of some of the USP Zf-UBD constructs. As a continuation in my quest to try and crystallize Read More …
Manuscript finished! Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems
Now that I’ve got my biophysical screening assays developed (19F NMR, SPR), as well as my hit expansion campaign started for small molecule inhibitors against the USP5 Zf-UBD, I need to be able to test if these small molecule inhibitors will antagonize the catalytic activity of USP5. It has been speculated in literature that the Read More …
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1495129. Apologies for the long delay between this post and my last post, it really has been quite some time! As postdocs, we generally have several projects running simultaneously and we have to split our time between these projects. I am no Read More …
Molecular dynamic simulations are a powerful tool for modeling biomolecular systems such as ligand binding to proteins. In a previous post I was able to get co-crystal structures of ligands which bind to the USP5 zinc finger ubiquitin binding domain (Zf-UBD). In order to explore the structure activity relationship (SAR) of the chemical series, so Read More …
Hello again! My previous post discussed how I finally had a specific antibody to EZH1 in a cell line (HEK293). As a follow up, I wanted to determine if we could detect EZH1 expression in patient cells. I’m happy to report I was able to detect the EZH1 protein in patient cells (Figure 1). Experimental Read More …
Huntington Society of Canada 2018 Conference in Kelowna
X-ray crystallography is a tool used for the structural determination of proteins and biological molecules. My specific project focuses on the zinc finger ubiquitin binding domain (Zf-UBD) of USP5; however, USP5 is just one of many USPs that contain a Zf-UBD. Table 1 shows a list of the USP Zf-UBDs and their available structures in Read More …
Biophysical investigation of HTT and HTT-HAP40 using DSLS and SAXS
