A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant NSD3 PWWP1 for further research. You can read more details on Zonodo in link below: https://doi.org/10.5281/zenodo.4115939
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant NSD2 PWWP1 for further research. You can read the purification protocol on zenodo.
Production and purification of recombinant SARS COV-2 RBDSD1-Fc protein from a transiently transfected EXPI293 mammalian cells. Expression plasmid for CoV-9-RBDSD1, residues of RBD R319−S591 with a C-terminal HRV3C protease cleavage site, a monomeric Fc tag and an 8XHisTag was generously gifted by Dr. McLellan’s lab (1). Secreted proteins were purified from a suspension culture cell Read More …
Over the last few months, we have all become familiar with the COVID-19 global pandemic. The coronavirus is “decorated” with the Spike (S) protein, one of the 25 proteins of SARS-CoV-2 that are encoded in the viral genome. The infection starts with the attachment of the virus to the cell membrane to enter host cells. Read More …
RBBP4 and RBBP7 (RBBP4/7) are highly homologous nuclear WD40 motif-containing proteins widely implicated in various cancers and are valuable drug targets. Below is the link to the expression and purification of the protocol for RBBP7. RBBP7 protein is used to develop drug screening assays towards the discovery of novel cancer therapeutics. https://zenodo.org/record/4086006#.X4ckLWhKi70
RBBP4 and RBBP7 (RBBP4/7) are highly homologous nuclear WD40 motif-containing proteins widely implicated in various cancers and are valuable drug targets. Below is the link to the expression and purification of the protocol for RBBP4. RBBP4 protein is used to develop drug screening assays towards the discovery of novel cancer therapeutics. https://zenodo.org/record/4085998#.X4cfCmhKi70
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant BIO NSD2 PWWP1 for further research. https://zenodo.org/record/4088510#.X4cUdWhKi70
The NSD protein family has been implicated in cancers, Therefore they are considered as potential targets for cancer drug discovery. Here is the Expression and purification protocol of recombinant BIO NSD3 PWWP1 for further research. https://zenodo.org/record/4088451#.X4cd6WhKi70
In my previous post 19, I showed how we assessed the druggability of the remdesivir-binding site of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) and determined the residues lining that site. In this post, we analyze the genetic diversity of RdRp catalytic site across Alpha- and Betacoronavirus entries from UniProt and among SARS-CoV-2 samples. In the context Read More …
Previously we reported our analysis on the structural diversity of binding pockets found on the SARS-CoV-2 main protease, methyltransferase, and papain-like protease, macrodomain. We then shifted our focus on the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp). SARS-CoV-2 is a positive-strand RNA virus, depending on its multi-subunit machinery to replicate its RNA. The catalytic subunit of RdRp Read More …
