Non-hormonal Contraception

Co-expression and purification of recombinant hOvastacin and hFetuin B in insect cell sf9

Posted onAuthorYufeng PanLeave a comment

hFetuin B(1-382)-Fc-6xHis and hOvastacin(1-431)-twinStrep purification: Nickel & Strep-Tactin®T 4Flow® high capacity FPLC column For relevant background please see relevant page: Assessing Ovastacin and Fetuin B as a Non-Hormonal Contraceptive Targets  (https://openlabnotebooks.org/assessing-ovastacin-an-fetuin-b-as-a-non-hormonal-contraceptive-targets/) Donor Plasmid: pFastBac-Dual vector was used for the dual expression of hOvastacin-twinStrep (driven by polyhedrin promoter) and hFetuin B-Fc-6xHis (driven by p10 promoter). Recombinant Read More …

One year of ABHD2 – Where are we now?

Posted onAuthorOliver ArnoldsLeave a comment

The Recap It’s been one year since the last update on the α/β hydrolase domain 2 (ABHD2) as a target for non-hormonal contraception (see my previous post here). The major challenges were the production of ABHD2 and development of a reliable activity assay to screen and quantify inhibitory compounds. At that point 14 different constructs Read More …

PLCζ1: a promising target for non-hormonal contraceptives

Posted onAuthorEve CarterLeave a comment

The Structural Genomics Consortium (SGC) has been funded by the Bill & Melinda Gates Foundation to research Women’s and Children’s Health, focusing on the advancement of drug discovery in reproductive biology and disease, child development, and childhood diseases. The SGC plans to generate protein reagents and chemical probes for several potential drug targets for new, Read More …

PATL2: A Potential Contraceptive Target August 2023 Update

Posted onAuthorMadison EdwardsLeave a comment

PATL2: A Potential Contraceptive Target A promising protein target for NHCs is Protein Associated with Topoisomerase II Homolog 2 (PATL2), a highly conserved, oocyte-specific mRNA-binding protein that represses translation1. For consanguineous families, the inheritance pattern for PATL2 is recessive; thus, infertility is often caused by homozygous mutations in the gene2. Although some mutations in PATL2 Read More …

Recombinant Expression and Purification of ABHD2 from E. coli

Posted onAuthorMadison EdwardsLeave a comment

His6-FLAG-ABHD233-425 (PBC042 A11) Purification: Nickel & Size Exclusion Chromatography  This post is also available at: Recombinant Expression and Purification of ABHD2 from E. coli | Zenodo DOI: 10.5281/zenodo.7696914 For relevant background please see relevant page: A promising target for non-hormonal contraception – The a/b Hydrolase Domain 2 | Zenodo Expression of ABHD2 For each 1.8 Read More …

Phospholipase C zeta 1 (PLCζ1): a promising target as a non-hormonal contraceptive

Posted onAuthorEve CarterLeave a comment

Background The Structural Genomics Consortium (SGC) has been funded by the Bill & Melinda Gates Foundation to research Women’s and Children’s Health, focusing on the advancement of drug discovery in reproductive biology and disease, child development, and childhood diseases. The SGC plans to generate protein reagents and chemical probes for several potential drug targets for Read More …

Characterization and Assessment of KCNU1/SLO3 as a Potential Non-hormonal Contraceptive Target

Posted onAuthorMadison EdwardsLeave a comment

Characterization and Assessment of KCNU1/SLO3 as a Potential Non-hormonal Contraceptive Target Introduction Project Proposal The Structural Genomics Consortium (SGC) has recently embarked on its first research project in reproductive biology as a part of the SGC’s new open science Women’s and Children’s Health Program (WCHP). This program, which was generously funded by the Bill & Read More …