Hello again! Here is my first attempt to find an EZH1 antibody. I have mainly focused on EZH2 in the PRC2 complex as the methyltransferase responsible for the H3K27me3 mark. However, EZH1 can functionally substitute for EZH2 in PRC2. We have a good antibody to detect EZH2 levels in patient AML samples. However, for some samples that we see detectable levels of H3K27me3 mark but no EZH2 expression, we suspect that EZH1 might be substituting for EZH2. We would like to get a good EZH1 antibody to determine expression levels of this protein in patient samples. I tested three antibodies for EZH1 using western blot, and despite some initial results of detecting a potential band for EZH1 in brain tissue with an Active Motif antibody (Figure 1A), we were unable to see it in patient cells (Figure 1B). Full experimental detail can be found on Zenodo. Also listed in my experimental details is our plan moving forward to obtain an EZH1 antibody!
Figure 1: Western blots using Active Motif EZH1 antibody (green) and actin (red) loading control in (A) brain lysate, (B) AML cell line (UCSD-AML1) and patient sample (151077)we expect would express EZH1. We detect a faint band of the expected size (~85kDa) in (A) but do not detect any band in (B).