A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
In my last post, I told you about my initial results soaking follow-up compounds into preformed HAO1 crystals. In order to generate more crystals (and so more structures), I purified some new protein (I’ll call it ‘batch #2’ for simplicity). However, this second batch, when crystallised, did not take up any of the soaked in Read More …
Good morning and how is everyone doing, enjoying that first cup of tea or coffee of the morning I hope. Myself, I am a tea person, always have been, my Irish nature, we drink it by the liter. Can’t beat a good of tea in the morning to revive oneself. And if it’s the afternoon Read More …
More forgotten experiments revived by my year-end report. Huntingtin chromatin retention is reduced in PARP KO cells, but in frustration over some confusing results, I overlooked this important data. Lesson: pause and look at the data before rushing on! All the details and links to Zenodo deposits can be found on the Truant lab website.
Background: Aside from determining the optimal numbers of DIPG cells to be seeded for viability assay, it is also important to validate that measurements using CellTiter Glo are in agreement with other methods of determining cell viability. Experimental design: I chose to compare the EC50 values of M4K2009 on HSJD-DIPG-007 when measured using different methods. Read More …
A short report on a successful method of detecting poly ADP ribose by immunofluorescence has now been posted on the Truant lab website.
Good morning and happy Monday, no, not feeling it. Yes, I agree, I hate Mondays too. I never fully understood why the day existed except to be worse then Tuesday, and don’t even get me started on Wednesdays. Anyway, I digress, I trust everyone had a lovely weekend, here in Montreal the sun was shining, Read More …
Hello there, Is anyone out there, can you hear me, are you still there. Oh, good you are there, hello. I have to start with a Mea Culpa, for all those wondering where Tom was, its been 3 months why isn’t he writing a new post, has he forgotten about us, well no I didn’t and I Read More …
As soon as possible I’m hoping to use CRISPR/Cas9 genetic editing to make sets of DIPG patient cells that differ only in one gene (either with/without the whole gene or with/without mutations of interest). This will allow me to make far more accurate comparisons of behaviour between the cells in relation to specific genes or Read More …
We are getting close to the midpoint of the summer and my project is well underway. With another week passed, I have more purifications to share. Again, I was attempting to purify proteins we think might interact with the huntingtin protein in neuronal cells. This time around was a little less successful than previous posts, Read More …
Hi Everyone, This is the first time I am going to share with you my research and I will start with the project I am currently working on, which is the development of cellular HDAC11 target engagement assay. HDAC11, the only member of class IV HDAC subfamily, was believed to be responsible for the deacetylation Read More …
