Transfectability of different patient-derived DIPG and Glioblastoma cell lines

Whether or not a cell line can be transfected efficiently is an important factor to consider when designing experiments. Often times scientists would like to deliver into the cells DNA or RNA with the instructions to produce specific proteins or to delete away specific proteins. Transfection via liposome (eg: Lipofectamine 2000) is a relatively simple and direct method of delivering nucleic acids into the cells compared to electroporation or transduction via viral vectors.

EGFP signal in different cell lines 24 hours and 48 hours after transfection using Lipofectamine 2000

For more experimental details, please refer to Zenodo.

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