Summer travels showcasing the huntingtin protein toolkit
Summer travels showcasing the huntingtin protein toolkit
Huntington’s disease
More Purifications
Back again. Two posts in one month! I know its crazy, but I’m just excited to tell you about the progress that is being made on this project. First things first, purifications. This week I have four more to tell you about. The first is GFP, and unlike all of my other posts, this is Read More …
More Protein Purification
Hello again! It has been a while since my last post but that is not for lack of data. Over the past month I have been purifying like crazy, adding more proteins to out bank of possible interaction partners. As we are now in the final month of the project, I want to take this Read More …
Project Overview: Overexpressing HTT in neuronal cells
My main project revolves around the E3 ligase UBE3A (I will be writing a project overview for this later), but I am also continuing some work in the same vein as Jolene Ho’s work looking at role of HTT in neurological disorders. HTT encodes a protein called huntingtin whose function is not known but appears Read More …
Purification of the HTT C-HEAT domain
I am very excited to write my first post in open notebook. My post on Zenodo summarizes the results of my first ever purification of a huntingtin (HTT) domain. I was able to purify successfully the HTT C-HEAT domain encompassing residues 2088-3144. Figure 1. Anti-FLAG purification of the HTT C-HEAT domain. Eluted protein is seen Read More …
Project Overview
I joined the group of Dr. Cheryl Arrowsmith 2 months ago. Currently, I am working on the Huntington’s disease project along with Dr. Rachel Harding and Jacob McAuley. During these past two months, I have been learning how to purify huntingtin (HTT) protein and overall getting up to date with the project. A brief introduction Read More …
Huntingtin chromatin retention in PARP knock out cells
More forgotten experiments revived by my year-end report. Huntingtin chromatin retention is reduced in PARP KO cells, but in frustration over some confusing results, I overlooked this important data. Lesson: pause and look at the data before rushing on! All the details and links to Zenodo deposits can be found on the Truant lab website.
Detecting poly ADP ribose
A short report on a successful method of detecting poly ADP ribose by immunofluorescence has now been posted on the Truant lab website.
Purification of putative huntingtin interacting proteins UBE2K, HAP1, NEDD4L
We are getting close to the midpoint of the summer and my project is well underway. With another week passed, I have more purifications to share. Again, I was attempting to purify proteins we think might interact with the huntingtin protein in neuronal cells. This time around was a little less successful than previous posts, Read More …
The Year in Review
A summary of the Berman Topper second year-end progress report, with links to the full report, are now posted on the Truant lab website.