Biophysical investigation of HTT and HTT-HAP40 using DSLS and SAXS
Biophysical investigation of HTT and HTT-HAP40 using DSLS and SAXS
Huntingtin
Generating HTT Knockdown Models
The experimental set-up and results covered in this post can be found here: doi.org/10.5281/zenodo.1420537 I was previously using an HTT overexpression model to look at any changes in proteins related to HTT or its function. Unfortunately, none of the related proteins I looked at showed any significant change in protein levels with HTT overexpression. There Read More …
Is huntingtin a PAR-binding protein?
Is huntingtin a PAR-binding protein?
Searching far and wide for proteins that bind huntingtin
Searching far and wide for proteins that bind huntingtin
Expression and purification of the HTT-HAP40 complex with expanded polyQ (Q54)
Expression and purification of the HTT-HAP40 complex with expanded polyQ (Q54)
Checking Disruption of Proteins of Interest with Overexpression of HTT
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1323902 As I’ve been having problems overexpression HTT properly in my cancer cell lines, I decided to continue looking at other proteins of interest using our best overexpression model so far, which is in HEK293T cells. Although it may not be a cancer Read More …
A closer look at the hits generates 2 new hypotheses
A closer look at the hits generates 2 new hypotheses
The ongoing quest for huntingtin interaction partners
The ongoing quest for huntingtin interaction partners
Starting new projects in huntingtin structural biology
Starting new projects in huntingtin structural biology
Overexpression of Expanded HTT in Brain Cancer Cell Lines
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1292884. A few posts back, I tried expressing HTT with different lengths of Q repeats in mammalian cells using baculovirus (read more here). However, expression was not very good, especially for HTT with longer Q repeats (have a look at these results here). Read More …