A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
I’ve been working recently on purifying some proteins for use in future experiments and wanted to explain a little bit about what I need each construct for as they’re all for different things. Purification, as always, is a time consuming business but vital for any biophysical experiment that I do given all my work is Read More …
Man, it has gotten cold and windy, its only been a month, and already we have gone from +25 degree sunshine, to overcast, sub-10 degree temperatures, with rain, true Irish weather. The joys of Fall as winter approaches. I hope everyone is doing well, I enjoyed my short hiatus from the notebook, but am now Read More …
As part of SGC-UNC’s scaffold hopping strategy to identify new series of compounds active against CaMKK2, we recently submitted a select number of compounds differing in hinge-binder for CaMKK2 enzyme inhibition assay. The hinge-binders included thienopyridine, furopyridine, quinoline, thienopyrimidines, pyrimidine, N-methyl azaindoles and quinazoline, Figure 1. Others are azaindoles, triazolopyridazine, pyrazolopyridine, imidazopyridine etc. Figure 1: Read More …
Recombinant protein expression is a prominent molecular technique for structural biology studies. For expressing recombinant proteins, a host organism is utilized as protein production factories. To obtain the desired recombinant proteins, we first design a recombinant DNA, which involves the information of protein to be produced. Then, we clone the desired DNA fragments into a Read More …
Hello folks, another day another deposition. That makes it sound horribly commonplace but actually Ros put in immense effort into crystallising and solving these proteins and it’s my job just to add the final polish (fix those last stubborn geometry errors), give it a once over to make sure there’s nothing obviously wrong, and whip Read More …
It’s being a while since I wrote my first post. I have been busy in the lab trying to isolate monomeric and stable samples of the N-HEAT domain just as we demonstrated with the C-HEAT domain (and by Dr. Rachel Harding). In this post, I summarize the results of the first round of purification of Read More …
One of the major findings of the HDSA-funded project to find huntingtin interactors relevant DNA repair was that many proteins that interact with huntingtin are modified by poly ADP ribose (PAR). Previous posts have described our hypothesis that huntingtin binds poly ADP ribose, our preliminary evidence that it does so, frustrating attempts to understand why it Read More …
One of the big aims of the M4K project was to look at the structure of potentially interesting molecules bound to the protein ALK2 and use that to try and design better molecules that might be useful in the treatment of the rare childhood brain cancer, DIPG. Ros did a lot of work on this Read More …
In my previous post I mentioned that there is controversy if HDAC11 can deacetylate histones. I decided to look at the effect of overexpression of HDAC11 on exogenous histone acetylation in cells. In my preliminary results I noticed that exogenous GFP-tagged histones, one day post transfection, have very low levels of acetylation; therefore I decided Read More …
I’ve been getting my stocks in order recently and catching up on some other work which has been both busy but not the most thrilling in terms of experimental results. As such I’ve not had much time to write up a blog post so I’ll try and get back on top of that although it’s Read More …
