A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
Hi all, it’s been a while since my last post; I’ve been busy in the lab purifying new protein constructs, developing a new assay and expanding the chemical series of my weak binding hits for USP5 ZnF-UBD, so there will be a couple of posts coming your way in the following weeks. This week, my Read More …
I joined the group of Dr. Cheryl Arrowsmith 2 months ago. Currently, I am working on the Huntington’s disease project along with Dr. Rachel Harding and Jacob McAuley. During these past two months, I have been learning how to purify huntingtin (HTT) protein and overall getting up to date with the project. A brief introduction Read More …
We are getting close to the midpoint of the summer and my project is well underway. With another week passed, I have more purifications to share. Again, I was attempting to purify proteins we think might interact with the huntingtin protein in neuronal cells. This time around was a little less successful than previous posts, Read More …
Hi Everyone, This is the first time I am going to share with you my research and I will start with the project I am currently working on, which is the development of cellular HDAC11 target engagement assay. HDAC11, the only member of class IV HDAC subfamily, was believed to be responsible for the deacetylation Read More …
As the summer continues to progress, so does my project. After the last round of purification, detailed in my previous blog post, I am ready for another round. During this purification, I will be working with SH3G3 and PACN1. PACN1 is responsible for cytoskeletal remodeling through regulation of several other proteins, as well as neuronal Read More …
As explained in my previous post [https://openlabnotebooks.org/2714-2/], my goal is to test whether structure-based approaches can guide the design of PROTACs. As a first step, I am evaluating whether protein-protein docking tools can accurately predict the interface between an E3 ligase and its target, a question recently explore by Drummond and Williams.1 This step is Read More …
To start off my project at the SGC, my supervisor, Dr. Rachel Harding, and I produced and purified three proteins. These proteins were RAB1A, RAB1B, and MECP2. Both RAB1A and RAB1B have functions in the initiation of autophagy, which is a cellular system that helps clean out old debris and malfunctioning parts. MECP2 is a Read More …
You may recall I was in Montreal for the last three months doing an internship with Molecular Forecaster Inc. (MFI), a computational chemistry company. I’m back in Toronto from my internship, ready to jump back into my research, but first I’ll summarize the virtual screening work I did at MFI for USP5. In a previous Read More …
The molecular blueprint of the living organisms, deoxyribonucleic acid (DNA), is often wrapped with a family of proteins, called histones, in mammalian cells. The functions of histones are vast, including the protection of DNA, and most importantly, the regulation of gene expression. However, the details of gene regulation function of histones are not clearly understood, Read More …
Huntington’s disease (HD) is a progressive neurological disorder caused by a mutation in the HTT gene which encodes for the huntingtin protein. The current understanding of the function of HTT is limited, and there are only a few sources that indicate that it likely plays a critical role in several intracellular processes such as Read More …
