A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
A belated Happy New Year everyone! It has been a good while since my last post so I wanted to update you on what I have been spending 2019 on so far. After identifying some really promising follow-up compounds in my activity assay, the next step was to find out how they bind to HAO1, Read More …
The idea for split-intein based SCN1A reconstitution started when I first learned of the split-Cas9 system. Long story short, packaging Cas9 with a guideRNA sequence into an AAV vector is pushing the packaging capacity of AAV vector. To circumvent this issue, the authors design a split-Cas9 system where the coding sequence for Cas9 is split Read More …
I thought I’d spend a blog post just talking about the various paths of my research and try and tie it all together. A lot of my experiments do dot around these various arms of investigation and so one experimental post doesn’t necessarily tie in directly with the previous one or the next one and Read More …
Hello fellow scientists and open lab notebook readers! I’m Carolyn Marks and I’m working in the ALS-RAP partnership with Susanne Gräslund, who leads the Recombinant Antibodies Group at SGC-Karolinska in Stockholm, Sweden. SGC-Karolinska has had a long-standing collaboration with Helena Persson Lotsholm, Director of the Human Antibody Therapeutics Facility within the Drug Discovery and Development Read More …
Optimisation of HTT-HAP40 purification using heparin affinity chromatography
In a previous post, I described using hit expansion tools such as commercial substructure searches, docking, and free energy perturbation (FEP) simulations to explore the structure activity relationship (SAR) of the chemical series focused on the Zf-UBD of USP5. The first batch of commercially ordered compounds arrived just before the Christmas holidays. I decided to Read More …
Further down the line I want to be able to test the activation of the BMP signalling pathway downstream of the ALK2 receptor. For this I plan to use the alkaline phosphatase gene as a readout – it’s a well known gene activated downstream of BMP signalling so a lot of easy to use assays Read More …
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.2541053. A lot has happened in the past few months and it is with mixed feelings that I am finishing up my postdoc here at SGC! I feel very privileged to have been part of the great science going on at SGC Read More …
Following an early commissioning stage, XChem crystallised its core xray collection protocol as 180 degrees rotation in 60 seconds, which provides PanDDA with good datasets. But what has never been fully characterised is the lowest dose per crystal required for PanDDA to work. XChem now has an established hit rate profile where, based on our Read More …
Hello everyone and happy New Year! I really, really wanted to end the year on a high note in December before I went off on holiday, but as it turned out, I didn’t have enough time to set up 18 plates and write a blog post on the last day. So I’ll just have to Read More …
