A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1253365. Recently, I helped run some Western blots for Rachel Harding, a fellow postdoc and open notebooker at SGC (read more about her work here: http://labscribbles.com/), for a publication on a toolkit to enable people to more easily purify and study HTT (you Read More …
Full-length huntingtin protein aggregation and first attempts to co-purify HTT-HAP40 from insect cells
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1233602. Lately, I’ve been helping Rachel Harding, a fellow postdoc at SGC and one of the pioneers of the Extreme Open Science Initiative at SGC, with some HTT experiments. She is also working on HTT, and has in fact been working on Read More …
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1218375 The etiology of HD is the expansion of Q repeats or CAG repeats in the HTT gene, which produces the huntingtin protein. This causes improper folding of the protein and leads to protein aggregation in neuronal cells, which ultimately causes the Read More …
Next steps in the identification of ROS-related huntingtin protein-protein interactions
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1202292 In my previous post, I used baculovirus to overexpress HTT in mammalian cells. Although I managed to overexpress HTT, this HTT is actually FLAG-tagged, and very strangely, I could not detect FLAG! Let me first explain what a FLAG-tag is. FLAG is Read More …
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1172644 One of the main aims of my project is to understand the role of normal and mutant HTT in mammalian cells. To do so, I will be overexpressing HTT in mammalian cells using baculovirus. The use of baculovirus for protein expression Read More …
ROS-dependent huntingtin interactions in mouse striatal cells
Before I get into the details of my project and share my results, I would first like to give a brief background on HTT. What is HTT? HTT is a protein that is encoded by HTT, the gene, and it stands for huntingtin. This protein is heavily implicated in Huntington’s disease (HD), a disease Read More …
Hi everyone! As part of SGC’s Extreme Open Science program, I’m going to be giving live updates on my project on the role of HTT in neurological disorders. As this is my first blogpost, I’ll start by giving an overview of my project. The focus of my project is HTT and its role in neurological Read More …
