A growing team of groundbreaking scientists around the world are now sharing their lab notebooks online
…and analysing their activity! Following on from the work I talked about here I’ve been able to start looking at the influence the different phosphorylation sites have over the ability of ALK2 to activate SMAD1. Just coming through the SGC expression pipeline are several constructs containing mutations in various combinations of the sites known to Read More …
Well hello there, did you miss me. No, I didn’t get stuck on a roundabout for days, preventing me from writing my next blog. I was simply enjoying the Montreal sunshine and those umbrella-containing beverages. Whoever came up with the idea of an umbrella in a drink is a genius. The umbrella shades the drink, keeping Read More …
My main project revolves around the E3 ligase UBE3A (I will be writing a project overview for this later), but I am also continuing some work in the same vein as Jolene Ho’s work looking at role of HTT in neurological disorders. HTT encodes a protein called huntingtin whose function is not known but appears Read More …
I am very excited to write my first post in open notebook. My post on Zenodo summarizes the results of my first ever purification of a huntingtin (HTT) domain. I was able to purify successfully the HTT C-HEAT domain encompassing residues 2088-3144. Figure 1. Anti-FLAG purification of the HTT C-HEAT domain. Eluted protein is seen Read More …
Hi all, it’s been a while since my last post; I’ve been busy in the lab purifying new protein constructs, developing a new assay and expanding the chemical series of my weak binding hits for USP5 ZnF-UBD, so there will be a couple of posts coming your way in the following weeks. This week, my Read More …
Synopsis The four, structurally similar Mur ligases are essential for the survival of gram-negative bacteria and are therefore potential targets for new antibiotics. Crystallographic fragment screening may provide new avenues for inhibitor development. Background The rapid emergence of bacterial resistance to almost all clinically available antibiotics is one of the biggest healthcare challenges of Read More …
I joined the group of Dr. Cheryl Arrowsmith 2 months ago. Currently, I am working on the Huntington’s disease project along with Dr. Rachel Harding and Jacob McAuley. During these past two months, I have been learning how to purify huntingtin (HTT) protein and overall getting up to date with the project. A brief introduction Read More …
As an Irishman, I am well versed in roundabouts, we love them. Entering the roundabout, going around it and then hoping to get off it when its your exit. If you don’t exit, you just keep going round and around and around, well you get the drift. It reminds me of that great scene in Read More …
In the immortal words of Ron Burgundy “Its so hot… milk was a bad choice”. Man, it is a scorcher today, with a humidex of really humid, now I know how cells feel like living in an incubator. But when its hot, what better way to cool down then with an ice cold beverage (I Read More …
The Intelligent Assistive Technology and Systems Lab (IATSL) is a multi-disciplinary group of researchers with backgrounds in engineering, computer science, occupational therapy, speech-language pathology, and gerontology. Our goal is to develop zero-effort technologies that are adaptive, flexible, and intelligent, to enable users to participate fully in their daily lives. Our research primary focuses on older Read More …
