The ongoing quest for huntingtin interaction partners
The ongoing quest for huntingtin interaction partners
Huntington’s disease
Starting new projects in huntingtin structural biology
Starting new projects in huntingtin structural biology
Overexpression of Expanded HTT in Brain Cancer Cell Lines
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1292884. A few posts back, I tried expressing HTT with different lengths of Q repeats in mammalian cells using baculovirus (read more here). However, expression was not very good, especially for HTT with longer Q repeats (have a look at these results here). Read More …
Visualisation of Purified HTT Proteins (2)
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1253365. Recently, I helped run some Western blots for Rachel Harding, a fellow postdoc and open notebooker at SGC (read more about her work here: http://labscribbles.com/), for a publication on a toolkit to enable people to more easily purify and study HTT (you Read More …
Full-length huntingtin protein aggregation and first attempts to co-purify HTT-HAP40 from insect cells
Full-length huntingtin protein aggregation and first attempts to co-purify HTT-HAP40 from insect cells
Visualisation of Purified HTT Proteins
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1233602. Lately, I’ve been helping Rachel Harding, a fellow postdoc at SGC and one of the pioneers of the Extreme Open Science Initiative at SGC, with some HTT experiments. She is also working on HTT, and has in fact been working on Read More …
Overexpression of Expanded HTT
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1218375 The etiology of HD is the expansion of Q repeats or CAG repeats in the HTT gene, which produces the huntingtin protein. This causes improper folding of the protein and leads to protein aggregation in neuronal cells, which ultimately causes the Read More …
Next steps in the identification of ROS-related huntingtin protein-protein interactions
Next steps in the identification of ROS-related huntingtin protein-protein interactions
N-terminal vs C-terminal FLAG-tagged HTT
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1202292 In my previous post, I used baculovirus to overexpress HTT in mammalian cells. Although I managed to overexpress HTT, this HTT is actually FLAG-tagged, and very strangely, I could not detect FLAG! Let me first explain what a FLAG-tag is. FLAG is Read More …
Overexpressing HTT in mammalian cells
The experimental set-up and results covered in this post can be found here: 10.5281/zenodo.1172644 One of the main aims of my project is to understand the role of normal and mutant HTT in mammalian cells. To do so, I will be overexpressing HTT in mammalian cells using baculovirus. The use of baculovirus for protein expression Read More …